Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: A fat- and sucrose-enriched diet causes metabolic alterations in mdx mice

doi: 10.1152/ajpregu.00246.2022

Figure Lengend Snippet: Antibodies used in Western blotting of whole muscle extract from the diaphragm

Article Snippet: pmTOR S2448 , 2971 , Cell Signaling , Rabbit , 1:1,000 TBST , 1:2,000 TBST.

Techniques: Western Blot

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: A fat- and sucrose-enriched diet causes metabolic alterations in mdx mice

doi: 10.1152/ajpregu.00246.2022

Figure Lengend Snippet: Assessment of autophagy and lysosomal markers cell signaling using Western blots. A : in whole muscle extract, AMPK, ATG12-ATG5, p62, LC3A/B I, and LC3A/B II were increased as a main effect of disease in mdx diaphragm compared with C57. pAMPK T172, pmTOR S2448, and LC3A/B II/I were decreased as a disease main effect in mdx diaphragm compared with C57. B : in whole muscle extract, lysosomal markers LAMP1 and LAMP2 were decreased, and several different forms of lysosome function marker cathepsin B (CTSB) were increased as a main effect of disease in dystrophic diaphragms compared with C57. Note, the 26-kDa CTSB band was not detected (ND) in muscle from C57 mice. C : Sestrin2 (SESN2) was decreased by disease and increased by diet main effects in diaphragm from mdx when compared with C57. D : KEAP1 in nuclear fraction indicates nuclear accumulation with disease. For all panels, representative sample blots and Ponceau S stains are included. Main effects (dis, disease; diet; and int, interaction) are indicated above each graph. Significance established at P < 0.05 for post hoc tests from two-way ANOVA. Different lowercase letters indicate differences between groups ( n = 6 mice/group for all groups; identified outliers were removed according to our a priori criterion).

Article Snippet: pmTOR S2448 , 2971 , Cell Signaling , Rabbit , 1:1,000 TBST , 1:2,000 TBST.

Techniques: Western Blot, Marker